The 4th International Conference on Nursing

Author :

Akhiyan Hadi Susanto

Affiliation :

School of Nursing, Universitas Brawijaya, Malang, Indonesia

Abstract :

Introduction Genetic variant G-152A of angiotensinogen (AGT) promoter is thought to affect AGT gene transcription and angiotensinogen levels. Angiotensinogen is an important substrate for renin in the RAS that is finally converted into angiotensin II that plays a key role on the occurrence of hypertension. However, the studies that discuss the single nucleotide polymorphism (SNP) G-152A of AGT gene are still limited, especially in Indonesian hypertensive patients. Methods This study was designed to detect the SNP G-152A of AGT and reported its serum angiotensinogen levels. The variants were identified in 62 patients with essential hypertension by PCR and further identified by automated sequencing. Serum samples were collected to analyze the angiotensinogen levels by a sandwich ELISA. Results The data showed that the AGT promoter in our patients had genetic variants -152G (G allele/GG genotype) and -152A (A allele/AG genotype) with a frequency of 0.92 : 0.08, respectively. There were no patients with AA genotype. In the analysis of serum angiotensinogen levels (mean ± SD), it was found that A allele had an angiotensinogen level of 371.30 ± 69.92 (ng/mL) and G allele was 343.53 ± 74.95 (ng/mL). The A allele had slightly higher angiotensinogen level than G allele with no significant difference (P=0.437). Conclusions Thus, in our study is found a genetic variant G-152A of AGT gene represented the GG, AG genotypes and it has higher frequency of G allele. Further research is still needed to determine that G/A allele directly affects on the occurrence of essential hypertension.

Keywords :

SNP G-152A; Angiotensinogen gene; Angiotensinogen Levels; Essential Hypertension